Microbial culturing—a technology over 100 years old—is the primary diagnostic tool for most infections, including prosthetic joint infections. Annually, ~1 million U.S. patients undergo joint replacement surgery, ~2% of these cases will develop an infection at some point. This complication often requires two additional surgeries and months or years of intensive antibiotic treatment. To increase positive identification by culture-based assays, antibiotic treatment is withheld for days to weeks. Despite this effort, ~15% of cultures end with a negative result, leaving little to no information to guide decisions between broad spectrum or targeted antibiotic treatments.
HSS is the largest orthopedic surgical center in the U.S. (ranked #1 by U.S. News and World Reports) with more than 10,000 total joint replacements surgeries each year and serves as a major referral center for complicated cases such as infections. Over the last year the Donlin lab along with the HSS ARJR and CJRC orthopedic teams and external collaborators have established a streamlined collection and clinical metagenomic sequencing program for joint infections. Together, we have demonstrated that this approach holds outstanding promise as a diagnostic tool in joint infections.
HSS is the largest orthopedic surgical center in the U.S. (ranked #1 by U.S. News and World Reports) with more than 10,000 total joint replacements surgeries each year and serves as a major referral center for complicated cases such as infections. Over the last year the Donlin lab along with the HSS ARJR and CJRC orthopedic teams and external collaborators have established a streamlined collection and clinical metagenomic sequencing program for joint infections. Together, we have demonstrated that this approach holds outstanding promise as a diagnostic tool in joint infections.
Publications
Sequencing of Circulating Microbial Cell-Free DNA Can Identify Pathogens in Periprosthetic Joint Infections
Journal of Bone and Joint Surgery
Adriana P Echeverria, Ian S Cohn , David C Danko, Sara Shanaj, Lily Blair , Desiree Hollemon , Alberto V Carli , Peter K Sculco , Carine Ho , Galit Meshulam-Simon , Christine Mironenko , Lionel B Ivashkiv , Susan M Goodman , Alexandra Grizas , Geoffrey H Westrich , Douglas E Padgett , Mark P Figgie , Mathias P Bostrom , Thomas P Sculco , David K Hong , Matthew S Hepinstall , Thomas W Bauer , Timothy A Blauwkamp , Barry D Brause , Andy O Miller, Michael W Henry, Asim A Ahmed , Michael B Cross , Christopher E Mason, Laura T Donlin
Journal of Bone and Joint Surgery
Adriana P Echeverria, Ian S Cohn , David C Danko, Sara Shanaj, Lily Blair , Desiree Hollemon , Alberto V Carli , Peter K Sculco , Carine Ho , Galit Meshulam-Simon , Christine Mironenko , Lionel B Ivashkiv , Susan M Goodman , Alexandra Grizas , Geoffrey H Westrich , Douglas E Padgett , Mark P Figgie , Mathias P Bostrom , Thomas P Sculco , David K Hong , Matthew S Hepinstall , Thomas W Bauer , Timothy A Blauwkamp , Barry D Brause , Andy O Miller, Michael W Henry, Asim A Ahmed , Michael B Cross , Christopher E Mason, Laura T Donlin
Increased Staphylococcus aureus Nasal Carriage Rates in Rheumatoid Arthritis Patients on Biologic Therapy
The Journal of Arthroplasty
Goodman SM, Nocon AA, Selemon NA, Shopsin B, Fulmer Y, Decker ME, Grond SE, Donlin LT, Figgie MP, Sculco TP, Russell LA, Henry ME, Bass AR, Miller AO, Sculco PK.
| Data Set |
The Journal of Arthroplasty
Goodman SM, Nocon AA, Selemon NA, Shopsin B, Fulmer Y, Decker ME, Grond SE, Donlin LT, Figgie MP, Sculco TP, Russell LA, Henry ME, Bass AR, Miller AO, Sculco PK.
| Data Set |